To understand toxic responses in organisms, it is necessary to determine the absorbed quantity of a toxic agent in a laboratory animal after topical application of that agent. Therefore, we developed a reliable analytical method for the analysis of sulfur mustard (HD) and applied it to the measurement of cutaneous absorption of HD in swine. Six pigs (Yorkshire Cross) with six HD application sites each were used in each of two separate studies. HD, 400 uL, was applied to each site for 7 or 8 minutes in study 1, and for 10 or 12 minutes in study 2. Identical multicomponent adhesive templates were used on all 72 sites to dose each animal reproducibly. After reaching the desired time point, the templates were removed and placed in screw-cap glass jars along with 75 mL of n-hexanes. Our objective was to analyze in duplicate all n-hexanes samples using a gas chromatography (GC) procedure and to calculate cutaneous absorption amounts. A stock solution of HD in n-hexanes was prepared gravimetrically and used to make up standards for a 3-point calibration curve. Based upon our analysis of the samples and standards, we determined a range of absorption amounts between 8.2 % and 10.8 % of the total dose. We saw no significant difference in absorption amount between study 1 and study 2. The experimental error of the analytical method was acceptable, and variation in absorption amount could be the result of biological differences within the study population. This method development and the determinations of absorbed HD amounts are important contributions to a better understanding of the cutaneous partial-thickness HD burn model in swine.